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4 Matters to Know When Using Syringe Filter

The HAWACH syringe filter’s shell is made of high-pressure polypropylene (PP), which is resistant to high temperature, does not leak, does not require membrane replacement and cleaning, and save complicated and time-consuming preparations. That’s why it’s widely been used in many industries. The commonly used ones are Φ13μm and Φ25μm, that is, Φ13μm for sample size 2-10ml, and Φ25μm for sample size 10-100ml. Hawach provides PTFE syringe filter, Polypropylene syringe filter, and HEPA activated carbon syringe filter and others for your choiceand others for your choice.

Application of syringe filter

1) Filtration of high-temperature liquids and special chemical
2) Used for clarification and filtration of strong acids and alkalis.
3) Sample filtration, liquid sterilization filtration, clarification and particle removal filtration, gas sterilization filtration.
4)) Clarification and filtration of air or various gases.

Syringe filter can also be connected with a catheter and a peristaltic pump for positive pressure filtration, which can filter larger volumes of liquid.
Winstar Series Hydrophobic PVDF Syringe FiltersFiltstar Series Hydrophobic PTFE Syringe FiltersSterile Hydrophobic PTFE Syringe Filters

4 matters to know when using syringe filter


1. Storage

The HAWACH syringe filter has a wide range of uses, liquid and gas phase sample preparation; environmental testing; routine QC testing; removal of protein precipitates; dissolution testing, etc., which can be used in many aspects. Because it is a one-time, fast and efficient. Disposable experimental supplies are most afraid of contamination. Whether they are being put or taken, they must be stored in bags and sealed, and gloves and tools must be kept clean when taking them. Cross-contamination is the most difficult thing to deal with.

2. Operation force

Do not use too much force, so as not to break the filter membrane and lose the filtering effect. There are some sample solutions with complex matrices, especially those with incomplete removal of fat or protein, or emulsified solutions, which will be particularly difficult in the process of membrane passing, blocking the membrane and the filtration does not move. At this time, change it to a new one and filter it again. It’s just a failure of the experiment. Do it again. Prevent emulsification or add some sodium chloride to remove the emulsification. How to make the sample solution clear and free of suspended solids is the goal of your research.

3. Integrity test

Syringe filter filtration is usually a key step in operation, so the integrity test is very important. By doing so, it will help: Confirm the actual filtration pore size of the membrane; Check whether the filter is well encapsulated; Detection of damage; Confirm correct installation; Confirm that the filtration system reaches the verification performance.

4. Filter membrane

Strictly select the appropriate filter membrane. The sample solution is organic. The organic filter must be selected. For the water phase, use the special filter membrane for the water phase. There are marks on the filter membrane box and pay attention to the distinction. Like the 1:1 aqueous organic phase, both membranes are passed through, strictly controlling the entry of any impurities.

Filter membrane Characteristics
Nylon Natural hydrophilicity Resistance to strong alkalis and organic liquids No need to infiltrate before use uniform aperture, good mechanical strength
MCE High porosity, good interception effect Used for filtration of water-based aqueous solutions; Unresistant to strong acids, strong alkali solutions and most organic solvents
CA Natural hydrophilicity Low protein adhesion, suitable for aqueous solution processing No nitrate, suitable for groundwater filtration Uniform pore structure; wide pore size selection
PES High solvent recovery rate, small residual amount; high throughput Very high microbial filtering capacity; unique threaded interface design Low protein adsorption, low dissolution
PVDF Hydrophobic, non-moisture absorption, easy constant weight Heat resistance and repeated autoclaving Resistant to chemical corrosion and oxidation; threaded interface design
PTFE Excellent chemical resistance; Both hydrophilic membrane and hydrophobic membrane available
GF  Natural hydrophobicity Large flux; large amount of pollution Good mechanical strength

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09/30/2021