About the Operation and Use Notes of Syringe Filters

The housing material of the syringe filter is made of high-quality sanitary polypropylene material, with precisely designed structure to ensure smooth filtration, rationalization of the internal space, and very low residual rate.

HAWACH syringe filter

The key technical parameters for a syringe filter include: material, pore size, diameter, pre-filter and pre-filter material, maximum filter pressure and inlet and outlet type. The advantages of HAWACH syringe filter is various series(filstar, winstar, and purstar), which is convenient for operators to choose the most suitable one; the diameter ranges from 4mm, 13 mm, 25 mm, 33mm, 50mm, and the pore size ranges from 0.1 um to 10um(0.1μm, 0.22μm, 0.45μm, 0.65μm, 0.8μm, 1.0μm, 3.0μm, 5.0μm, 10μm), which can meet the filtration requirements of different volumetric capacities and different filtration purposes; there are 10 major membrane materials(Active Carbon, Nylon, PTFE/PTFE L, PES, PVDF, MCE, CA, GF, and PP) to meet the filtration needs of customers in different research fields. So you can choose Active Carbon syringe filter, hydrophilic PTFE syringe filter, PP syringe filter, and so on.

Sterile PVDF Syringe FiltersSterile Hydrophilic PTFE Syringe FiltersSterile MCE Syringe Filters

Due to the factory’s strict quality control, the syringe filter production has strict links such as raw material inspection, production process monitoring, and finished product inspection to ensure its consistant high quality. There are sterilization and non-sterilization types.

Non-sterilization means that syringe filter is not processed and retains its original form after it is produced, and is mainly used in places that do not have high requirements for the filtration environment. Sterilization means that syringe filters have been processed. For HAWACH syringe filters, they are all processed by gamma rays, suitable for sterile environments with extremely high environmental requirements, and there are more biological experiments.

As one of the most frequently used tools in the lab, do you know how to achieve filtering effect and the matters to be noted for syringe filters during use? HAWACH will provide your answers in the following.

How to operate the syringe filter to achieve the filtering effect?

The syringe filter can achieve the filtering effect by following these steps:

1. Before inhaling the sample, draw about 1 mL of air into the syringe. This can minimize liquid
2. Draw your sample into the syringe, and then inhale 1mL of air. Invert the syringe and wipe off the residue on the tip.
3. Use the Luer connector to connect the syringe to the syringe filter. Tighten gently to ensure a safe seal.
4. Take the filtered solution into syringe and then inject it to sample vial. Remove the syringe filter then, draw air into it and install it again. Filter out the residual sample by pressing the push rod to achieve sample recovery.
5. Note: Use a syringe with a volume less than 10 mL with caution. Because it is easy to generate enough pressure to burst the syringe filter.

What are the general precautions during the use of the syringe filter?

1. Disposable syringe filters have a wide range of uses, such as liquid and gas phase sample preparation; environmental testing; routine QC testing; removal of protein precipitates; dissolution testing, etc., can be used in many ways. It is most afraid of contamination. Whether they are placed or taken, they must be stored in bags and sealed. Keep gloves and tools clean when taking them. Cross-contamination is the most difficult thing to solve and the most difficult thing to deal with.

2. Do not use too much force, so as not to break the filter membrane and lose the filtering effect. There are some sample solutions with complex matrices, especially those with incomplete removal of fat or protein, or emulsified solutions, which will be particularly difficult in the process of membrane passing, blocking the membrane and the filtration does not move. At this time, operator should not be persistent. Change it to a new one and filter it again. It’s just a failure of the experiment and re-do it to prevent emulsification or add some sodium chloride to remove emulsification. How to make the sample solution clear and free of suspended matter is the goal of the research.

3. Strictly select the appropriate filter membrane. If the sample solution is organic, the organic filter must be selected. For the water phase, use the special filter membrane for the water phase. There are marks on the filter membrane box and pay attention to the distinction. Like the 1:1 water-phase organic phase, we need to go through both membranes to strictly control the entry of any impurities.

4. The filter membrane of the pigment must be verified by experiments, and the filter membrane that will not adsorb all the pigments should be selected. After the pigment sample solution passed through the filter membrane, the filter membrane turned red. After the sample was injected on the machine, it was found that there was no erythrosine at all, and it was known that the erythrosine pigment was adsorbed on the filter membrane.