The method of syringe filter sterilization:1. The general method is steam sterilization. Syringe filter Steam sterilization at 120°C for 30 minutes. During autoclaving, the steam should be prevented from directly rushing on the membrane in one direction, and the back of the membrane should be prevented from being damaged due to negative pressure generated by steam condensation.
Can syringe filters be reused? We do not recommend reusable disposable syringe filter. Because there is a possibility of cross-contamination with repeated use. Once a problem occurs, it is necessary to check whether the sample is contaminated. This will result in higher costs. When cleaning the syringe filter, the distilled water is firstly pumped repeatedly with a needle tube, followed by ultrasonic for about 20 minutes, then soaked in distilled water for more than 5 hours, then removed and dried at about 40 degrees. Don't bake the gaskets, just let them dry naturally.
The cleaning and disinfection of the micron syringe filters after use is the same as that of other high-pressure plastics, but polyethylene cannot be high-pressure.
A. The water is washed ten times and dried. We also use the gasket for drying. The discoloration has little effect. In general, set the oven temperature is usually set at 56 degrees because EDAT is often needed to be dissolved;
B. Soak in 1% hydrochloric acid is soaked overnight (less than 6h);
C. Water is repeatedly washed 10 times and then distilled water is washed twice, then the filter membrane can be installed under high pressure or dried for later use.
Do not soak the syringe filter cap in acid liquid, boil it for 20 minutes or soak the cap in NaOH for 6-12 hours. Install two filter membranes before packing. We need to pay attention to the membrane up or downside and make sure it's upside, when the filter membrane is installed. Slightly loosen the screw, place it in an aluminum box, sterilize it for 15 pounds for 30 minutes in a pressure cooker, and then dry it for later use. Please note that,when taking it out, tighten the screw immediately.
Soak the filter membrane in three steamed water for at least half an hour at high pressure. In addition, do not tighten the lab syringe when installing. The membrane has both sides, smooth face down, rough face up, so that the filters will save effort. Good for immediate use after high pressure. Filter membrane is good do not want secondary high pressure.
The filter membrane is generally put in two layers. The upper membrane was 0.4 micron, the lower 0.22 micron, and the two layers were 0.22 micron. Put the PTFE syringe filter into the PES filters after soaking for 10~20 minutes. After it is packed, wrap the syringe filters in a cloth syringe filter sterilization. Note that the membrane should be perpendicular to the ground so that it will not break even at high pressure. The autoclavable syringe filter should be soaked in distilled water for more than 3 hours or overnight, and then placed on the syringe filters screen, smooth face up. When you twist the syringe filters, you can just twist the airtight.
If you cannot tighten the syringe filters, lift the vent switch and wrap the outlet with sulphate paper. Wrap the strainer in kraft paper. The syringe filters should be tightened when using, but the force should be evenly applied.
Soak in dilute hydrochloric acid for about an hour. After the tap water is rinsed, soak in the double steam water overnight. After drying, moisten the syringe filters membrane face up, then stick it on and twist the double steam water, but do not tighten it too tightly. Fetch, then dry, it's ready to use. Note that if the syringe filter is not used up at one time, do not disinfect it twice to prevent the filter membrane from breaking.