Lab syringe filters have different pore sizes. Common ones are 0.2um and 0.45um. Most programs usually use a 0.45um filter. However, if there are very fine particles in the sample, it is wise to use 0.2um or even 0.1um particles. It is important to note that there are other ways to filter very small particles, such as a centrifugal filter, which may be a better choice.
Lab syringe filters also have a variety of materials used for filters. Cellulose acetate, PVDF, polyethersulfone and polyamide are just some of the common ones. In addition, they have specific compatibility, so be sure to choose carefully. The material will also affect the EFA or effective filtration area and will also affect the capacity.
When particles accumulate over time, they can clog the pores and eventually render the filter ineffective. This is why it is important to choose a lab syringe filters that can hold more liquid. Generally, a larger diameter filter has a higher hold-up volume. The retention is the amount of liquid remaining after using the filter. It is also recommended that smaller diameter filters should be used with rare fluids and expensive fluids.
Consisting of a filter medium and housing or holder that constrains and supports the syringe filter media in the sample's path, filters are good tools when you need removing particulates from samples in the lab.
We have three basic types of laboratory filters.
Membrane filters size rating of the membrane is determined the size of the pores. Centrifugal filters are suitable for separating such as protein or nucleic acid desalting and concentration. These devices drive the liquid through the filter through centrifugal force. And syringe filters which consist of a filter element and housing assembly are used when a sample must be filtered before entering a syringe.
To select the best filter for your process, ask yourself the following questions:
1. What are you going to filter?
2. What is the size and nature of the particles or molecules to be removed?
3. Chemical composition of your sample.
5. Suitable temperature.
More to be concerned upon your applications:
1. To achieve the separation, what pore size or nominal molecular weight rating is required?
2. Do you need a sterilizing filtration?
3. How quickly do you need to filter?
4. Pressure-driven or vacuum-driven filtration?
Now, your knowledge is improved to obtain accurate separation of your sample, mobile phase or other liquid.
When you are not concerned about low-concentration contamination and you are dealing with high-concentration samples: get a suitable filter. Inhale 1ml of air, and then sample in a sterile syringe. Pour out 1 ml sample in the waste container.
Discharge the remaining sample into a clean vial for storage. Push the newly inhaled air into the same vial. This step will push out the remaining liquid and reduce the hold-up volume. Dispose of the syringe and filter properly.
When you are concerned about contamination, the process is very similar, but there are other steps that need to be performed before taking a sample: Use suitable filters. Aspirate 1ml of air cushion, and then aspirate 10ml of blank matrix. Eject the substrate together with the air cushion into the waste container.
This may be the case with lab syringe filters, not only making them more and more popular in use, but also improving the process of filtering contaminants from various liquids used in the biotechnology industry.
Lab syringe filters are usually wheel filters with plastic bodies. Membrane is a filter used to effectively purify liquids and gases (such as nucleic acids, proteins, enzymes and peptides) for various industrial and laboratory purposes.
In addition to biotechnology-based industries, lab syringe filters can also be used in pharmaceutical, food and beverage, environmental and general laboratory industries. Lab syringe filters have also been found to be useful for HPLC sample preparation, routine analysis, food analysis, and environmental and biofuel analysis.
Lab syringe filters, especially certified ion chromatography and tissue culture filtration and purification of contaminants as described above. There are various other types of lab syringe filters, and each filter is distinguished based on the type of membrane, so it is more suitable for a specific type of fluid or liquid. Lab syringe filters have various pore sizes, and may have membranes made of hydrophilic or hydrophobic materials. The type of membrane you will need depends on the nature of the solvent or substance you are purifying.