Syringe filter is a kind of filter connected to the front of the syringe. When the experimenters deal with the chromatographic samples with syringe filter, they should pay attention to avoiding bringing impurities in the process of filtration.
When handling hydrophilic samples, the experimenters should select hydrophilic membranes, which has an affinity to water and is suitable for filtrating solvents in which water is regarded as a substrate. The available filter membranes are mixed cellulose esters, etc. When handling strong corrosive organic solvents, the experimenters generally use hydrophobic membranes, such as PTFE, polypropylene (PP) and other materials. When the samples are referred to the protein solutions, people will choose the membranes with low protein adsorption, such as PVDF membrane. And it is generally considered that PES membrane is more suitable for the filtration of solutions with low inorganic ions.
Besides the above factors we have mentioned, people usually consider about the volume of samples. Different volumes of samples mean choosing different sizes of syringe filters.
When syringe filter is used to treat chromatographic samples, it is necessary to avoid the introduction of other impurities in the filtration process. The appearance of this impurity may be that the shell material or filter membrane will drop particles and bring it into the filtrate. Therefore, the material of filter membrane and shell structure is very important. Different types of filter membranes are suitable for samples with different properties.
1) Hydrophilic samples: hydrophilic membranes are selected, which have affinity to water and are suitable for filtering water as matrix solution. The available filter membranes are Cellulose Acetate Syringe Filter, polyethersulfone (PES) Nylon and so on.
2) Strong corrosion erosion organic solvents (properties: transparent, colorless liquid): hydrophobic membranes, such as PTFE Syringe Filter, polypropylene (PP) and other material quality filter membranes, are generally used.
3) Protein solution: select low protein adsorption filter membrane, such as PVDF filter membrane. PVDF Syringe Filter
4) Ion chromatography: it is generally considered that the PES filter membrane is more suitable for filtration of a solution of a low inorganic ion.
In addition to the above factors, the volume of the sample shall be taken into account, and when the sample volume is less than 2 ml, a micro-filter with a diameter of 4 mm is selected; the sample quantity is between 2 and 10 ml, and a filter with a diameter of 13 mm is used; when the sample quantity is more than 10 ml, a needle type filter with a diameter of 25 mm is used.
Syringe filter provided by Hawach Scientific uses PTFE as source materials, which is an effective filter for solvent chemicals and other non-aqueous solutions. The chemical stability and the inert PTFE membrane with strong chemical compatibility are particularly suitable for filtration of the corrosive, strong acid and strong alkali chemical, and the ethanol and the water can be added so as to have certain hydrophobicity. Moreover, the product structure design is precise, the inner space rationalization, the residual rate is very low, and thus it ensures the filtration fluency and reduces the sample waste.
Hawach overcomes shortcomes and defects of the conventional syringe filter, and pursues more humanized design and higher quality to conform to the market needs and consumer satisfaction. In HPLC analysis, the packing size of chromatographic column is small and easy to be blocked by impurity particles, so Hawach syringe filter are designed to filter the sample and solvent in advance to remove particle pollutants and protect the instrument. Additionally, Hawach syringe filter can also be used in HPLC separation and IC analysis, which will greatly reduce the possibility of inorganic pollutants be introduced into sample pretreatment.
Different application fields call for different types of syringe filter. To meet the diverse needs of consumers, Hawach is available in three series of syringe filter, comprising sterile syringe filter, advanced syringe filter and standard syringe filter, such as Glass Fiber Syringe Filter. Sterile syringe filter can also be divided into sterile cellulose acetate syringe filters, sterile hydrophilic PTFE syringe filters and sterile hydrophobic PTFE syringe filters and so on.
This series of syringe filter is fit for common applications and sample volume; disposable hydrophilic PTFE syringe filter is suitable for aqueous and aggressive organic solvent-based solutions filtration and HPLC operation; advanced syringe filter can be widely used in gas filtration and sterile ventilation.