How to Use Syringe Filter?
Syringe filter is an important consumable for sample pretreatment during HPLC and IC analysis in the laboratory. It can filter the sample solution, remove particulate contaminants and protect the instrument. As a convenient and reliable filtration tool routinely used in the laboratory, the syringe filter is conducive to the simplification of experimental operation steps and can greatly improve the work efficiency. Therefore, it is very important to scientifically master the use methods and precautions of syringe filters.
The use of a syringe filter helps to better meet your analysis needs and the analysis quality, speed and reproducibility requirements. HAWACH was born to meet the routine analysis needs of your laboratory.
A variety of membrane types and syringe filters of different specifications.
- The integrity of the filter membrane and the uniform pore size ensure the effectiveness of the filter and ensure the analysis flow path will not block.
- The high cleanliness of the filter membrane and syringe filter housing ensures extremely low dissolution and will not contaminate the sample
Affect analysis results, ensure the accuracy of experimental results, and improve laboratory efficiency.
- The porous structure of the filter membrane ensures a high flow rate during filtration and improves filtration flux and laboratory efficiency
- Adapter Luer connector, matching with Luer connector syringe, easy to use, no leakage, to ensure laboratory environment
- Hydrophilic PTFE Syringe Filters, Hydrophobic PTFE Syringe Filters,sterile PP Syringe Filters, Syringe filter PES, MCE, PVDF, nylon, a variety of filter membranes and specifications to choose from, to meet your multiple application needs.
Syringe filter selection and usage suggestions:
The pore size of the syringe filter membrane is 0.22μm and 0.45μm. Among them, the 0.22μm can remove extremely fine particles in the sample and mobile phase, and can meet the requirements of 99.99% sterilization stipulated by GMP or Pharmacopoeia; 0.45μm pore size can filter Except for most bacterial microorganisms, it can meet the filtration of conventional samples and mobile phases, and can meet the general chromatographic requirements.
For chromatographic column systems using particles of 3 μm or larger, a 0.45 μm syringe filter or membrane can be used; for chromatography systems using particles smaller than 3 μm, or those involving microbial growth, a 0.22 μm filter is recommended.
Using syringe filters
1. The syringe filter has different membrane materials, and the different membranes materials have different applications. The filter membrane is suitable for sample filtration should be selected according to the your experimental needs;2. Connect the syringe filter to the needle, and tighten it gently to ensure a good seal;
3. The syringe filter is divided into two types: sterilization and non-sterile. The sterilized syringe filter needs to be pretreated before use. After the filter is installed, rinse the filter system with clean water, and then filter operate after cleaning;
4. Before inhaling the sample, it is necessary to suck about 1ml air into the needle, which can minimize the liquid residue;
5. The operation should be carried out according to the steps to ensure the recovery efficiency. First, suck the sample into the syringe, invert the syringe and clear all the residues at the top. Then filter the sample in the syringe and inject it into the sample vial, and finally remove the filter. Finally remove the filter, draw air into the syringe, reconnect the filter head, and push out any residual sample.
Precautions for using syringe filters
1. Do not use a syringe filter smaller than 10 cc, because the small-volume column tube may cause the pressure to exceed the upper limit, resulting in damage to the filter membrane or personal injury.2. Only for laboratory use, one-time use, non-renewable.
3. The filtrate at the beginning should be discarded. The volume is about the dead volume of the filter. Taking 25 mm as an example, discard the first 1mL filtrate; or pre-clean the filter with 1 to 2 mL of solution.