Main Classifications and Use Notes for Syringe Filter

Syringe filters are suitable for life science, pharmaceutical, environmental, biotechnology, food and beverage, agricultural testing laboratories and other fields, especially widely used in life science laboratories, for small volume samples (such as proteins, additives, buffers), reagents and drugs) for sterilization operations. The syringe filter housing is made of medical grade polypropylene, featuring one-time injection molding, high pressure resistance, no product leakage; easy-to-tear paper-plastic independent packaging.

Specifically, the syringe filters are widely used in liquid and gas phase sample preparation; environmental testing; routine QC testing; removal of protein precipitates; dissolution assays, etc., and can be used in many aspects. Because it is one-time, fast and efficient. Disposable laboratory supplies are most afraid of contamination. Whether it is stored or used, be sure to pay attention to the bag and seal, and ensure that gloves and tools are clean when using. Cross-contamination is difficult to solve and difficult to handle.

 Glass Fiber Syringe FiltersPP Membrane Syringe FiltersHydrophobic PTFE Syringe Filters

Main classifications of Syringe Filter

As we’ve discussed above, the syringe filter is used for filtration of particles and bacteria such as gas, oil, beverage, wine, etc., and can be used as the inspection of particles and bacteria. Syringe filter is DNA/RNase-free, pyrogen-free, endotoxin-free. It can be classified into different types based on different standards.

1. Filter membranes

The syringe filter can be divided into PTFE, PES, NYLON and PVDF and so on based on the filter membranes’ materials. The PTFE syringe filter is resistant to various solvents, suitable for various organic solvents and water system, with low solubility, large gas flux, high particle interception rate, good temperature resistance, strong acid and alkali resistance, non-stick, non-flammable, non-toxic, biocompatible and so on.

For the PES syringe filter, it features high mechanical strength and high chemical and thermal stability. The Nylon syringe filter has good hydrophilicity, which is not only suitable for aqueous solutions containing acids and bases, but also for organic solvents such as alcohols, hydrocarbons, lipids, phenols and ketones. Also, the PVDF syringe filter is frequently used, featuring chemical stability and inertia, and is suitable for chemically corrosive organic solvents, acid-base solutions, and sample preparation for liquid chromatography analysis. It is hydrophobic and can filter moisture from air and gases.

When using it, it must not be too hard, so as not to break the filter membrane and lose the original filtering effect. Some sample solutions with complex matrices, especially those in which fats or macromolecular proteins have not been removed, or emulsified solutions, will be particularly difficult to pass through the membrane, blocking the filter membrane, and the filtration will not move. Replace it with a new one and filter it again. Strictly select a suitable filter membrane. The sample solution is an organic phase, and an organic filter membrane must be selected. For the aqueous phase, a special filter membrane for the aqueous phase is selected. There are marks on the filter membrane box, so be careful to distinguish. Like the 1:1 water-to-organic phase, both membranes are filtered once to strictly control the entry of any impurities.

1. Interface form: female bayonet; formula socket.
2. Dimensions: 4mm\13mm\17mm\25mm\30mm\33mm\50mm, etc.
3. Filter layer structure: single-layer membrane structure; multi-layer membrane structure; capillary structure.

Precautions of Syringe Filter

1. When processing chromatographic samples, it is necessary to avoid the introduction of other impurities in the filtration process.
2. Do not use a syringe smaller than 10cc, because the small volume of the column may cause the pressure to exceed the upper limit, resulting in damage to the filter membrane or personal injury.
3. One-time use, non-renewable.
4. Part of the filtrate at the beginning should be discarded, and the volume is about the dead volume of the filter.

06/08/2022