Syringe Filters Used For Ventilation and Sterilization
During cell culture, cell contamination often occurs. Therefore, sterilizing the cell culture fluids ensures that they do not become a source of contamination. Autoclaving is not suitable for most liquids because it destroys key components of the culture medium and biomacromolecules needed for cell growth. Then, filtration becomes the ideal sterilization method.
When sterilizing filtration, 0.2 μm filters are always the best choice. Hawach is also available in a variety of pore sizes, including 0.1 μm, 0.45 μm, and 0.8 μm, 0.45 μm and 0.8 μm filters are often used for lysate clarification and prefiltration. As for the 0.1 μm filter, that is ideal for mycoplasma. It can ensure that mycoplasma will not enter your culture with serum or serum-containing medium to the greatest extent, and build a last line of defense against mycoplasma contamination.
Membranes are highly consistent, with small batch-to-batch variation, ensuring consistent analytical results. The low leaching content of this membrane ensures that you will not introduce new pollutants while filtering to remove unwanted particles, which is often referred to as "secondary pollution". Therefore, in addition to sterile filtration, it is also suitable for routine sample preparation, lysate clarification, magnetic beads/resin beads combined filtration, etc.