As we know in modern labs, particulates in extracts can damage instrument components, and lots of time and money for repair are followed. We can draw a conclusion that clean sample extracts while maintaining analytical instrumentation is very important. Even if it is not plugged, the slow accumulation of particles will influence the flow rates and result of the applications after all.
The syringe filters are good tools to remove particulates and clean-up the samples. The filter can fit onto the end of the syringe which contains the sample very easily. And the different membranes can be selected based on the characteristics of the solvent.
When we push the extract gently through the filter into a sample vial for injection, the damaging particulates can be removed from the final extract. If we use a syringe with a Luer lock tip, the connection can be more strengthened, standing higher filtration pressure. To make sure that the filter is not overloaded, the right choice of diameter depends on the sample volume. And the porosity depends on the applications.
Under strict manufacturing standards, the goal of Hawach is always to bring the highest quality syringe filters to you. To make the sample preparation easy and protect analytical equipment as well, Hawach syringe filters are the best choice, reliable, and cost-effective.
Hawach provided Cellulose Acetate Syringe Filters, Polypropylene Syringe Filters,0.22/0.45 um Hydrophilic PTFE IC Syringe Filters for your choose from. The syringe filters are mainly used for HPLC sample preparation, routine QC analysis, removal of protein deposits, dissolution determination, food analysis, biofuel analysis, environmental testing, liquid and gas sterilization filtratio, sample prefiltration, clarification and removal of particles and so on. One of the most important factors to consider for a syringe filter is the membranes. The membrane should be selected based on the sample. The filter membrane’s main function is to achieve separation and purification by trapping impurities from liquid or gas phase, such as particles or bacteria.
So, when choosing a filter membrane, we should consider these factors, such as the material, pore size etc.
The filter membrane is made of polymer chemical materials, and pore-forming additives are specially treated and then smeared on the support layer. Due to its easy use and fast speed, it is widely used in many fields, such as the food inspection, scientific research, environmental protection, chemical engineering and so on.
1. Filter membrane material (chemical compatibility)
When choosing a microporous membrane, the first thing to consider is chemical compatibility. Is the filter membrane resistant to acids, alkalis, organic solvents, etc.
2. The pore size of the filter membrane
3μm or larger particles, 0.45μm syringe filters are used for chromatographic column systems; for chromatographic systems using less than 3μm fillers, or chromatographic systems involving microbial growth, 0.22μm membranes are recommended. For difficult-to-handle turbid solutions, you can use a 1-5μm filter membrane for pre-filtration, and then use the corresponding filter membrane for continuous filtration.
Introduction to the different functions of different pore sizes of microporous membranes:
0.22μm: It can remove very fine particles in samples and mobile phases; it can meet the requirements of 99.99% sterilization specified by GMP or Pharmacopoeia.
0.45μm: it can filter out most bacteria and microorganisms; conventional samples and mobile phase filtration can meet general chromatographic requirements.
1-5μm: To filter larger particles of impurities, or for the pretreatment of difficult-to-handle turbid solutions, it can be filtered with a 1-5μm membrane first, and then filtered with the corresponding membrane.
Commonly used filter membranes include PTFE membrane, GF and Nylon membrane, PES and PVDF and so on. The PTFE membrane is chemically compatible and can filter almost all organic solvents and strong corrosive chemicals; Glass fiber pre-filtration membrane is used to increase the filtration rate and continuous filtration. Nylon membrane is used to filter alkaline solutions and organic solvents.
Click here to know Several Common Materials And Advantage For Syringe Filter